Super-Resolution fluorescence microscopy
(3 Hp, SK3514)
January 18th to 22th 2021
The course aims to provide all participants with training in the fundamental theoretical and experimental aspects of super-resolution microscopy (Nobel Prize in 2014).
Scientific tutorials will introduce fundamental and advance aspects of super-resolution fluorescence microscopy. Experimental demo-session will further deepen the understanding and applicability of super-resolution microscopy.
As the name implies, super-resolution fluorescence microscopy is a field in which novel optical approaches and single molecule sensitive fluorescence methods are applied to answer question in cellular biology via nanoscale biological imaging.
The course will be carried out at the national Science for life laboratory in Solna
Lectures / seminars:
Introduction to available super-resolution fluorescence microscopy techniques
(e.g. STED/PALM/SIM/RESOLFT/DNA-PAINT etc.) and their applicability.
Demo-sessions on commercial super-resolution microscopy platforms.
Registered as PhD-student and submission of access project proposal (link below).
Non KTH-students also need to fill in the KTH-admittance form (link below).
Literature study (1 university credit)
Laboratory work (1 university credit)
Examination (1 university credit)
Scientific articles (distributed)
Last registration date: 2021-01-07
After the course all participants should have acquired the following knowledge/skills:
Access project proposal form KTH-admittance form
In the case of an overbooked course (maximum 24 students) selection will be done on submitted project proposals (i.e. necessity of super-resolution imaging). The proposal form can be down loaded from below. When completed the form should be sent to firstname.lastname@example.org no later than 2018-01-10
Course Time Table
Week 1: January 11 - 15, 2021
Background on superresolution microscopy methods – reading review articles.
Deliver 3 personal questions on ‘want-to-know-more´ (understand) about each type of principal method SIM | PALM/STORM/PAINT | STED/RESOLFT.
Week 2: January 18 - 22, 2021
Lectures, webinars, demo and discussion sessions.
Week 3: January 25 - 29, 2021
Examination – to pass the course each participant hand in an ´improved´ project proposal, with know-how on possibilities and constrains acquired during the course.
Course Memo can be down loaded here:
The super-resolution fluorescence microscopy course ends with a take-home exam (project proposal writing) where each PhD-student addresses how to apply or further develop one or several techniques in their own research. The grade of the course is either pass or fail.