Super-resolution wide-field microscopy. The multicolor capability of 3D-SIM allows the imaging of several cellular components and the mapping of their relative positions in macromolecular complexes, enabling the study of their spatial relationship within the surrounding cellular contexts
With this approach the lateral and axial resolution increases by a factor of two beyond the classical diffraction limit
x, y ≈ 100 nm; z ≈ 300nm
An attractive feature of structured illumination for cell biological applications is the fact that standard dyes and staining protocols can be used and multiple cellular structures can be simultaneously imaged with optical sectioning in three dimensions
Up to four colors e.g. DAPI; Alexa488; Alexa568, Alexa647, or similar spectral dyes or fluorescent proteins.
Fixed cells and tissues