Light sheet microscopy
Light Sheet Fluorescence Microscopy permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.
The facility offers access to a commercially available Lightsheet Z.1 (Zeiss) microscope, allowing users to image live and optically cleared samples at unprecedented speeds. Collaborative project support is provided with sample mounting, image acquisitions, post-acquisition processing and analysis of the resulting data.
Light Sheet microscopy - live, fixed and optically cleared samples
Sample preparation and mounting
Post-acquisition processing - Image stitching, multiview fusion and deconvolution
Data analysis - Arivis Vision 4D and Imaris 8.x
Zeiss Light Sheet Z.1 including:
Dual side Illumination with pivot scan to reduce shadows and striping artifacts
CO2 and temperature control modules
Two sCMOS PCOedge cameras for simultanous dual colour acquisition
Morphogenesis, Embryogenesis and Organogenesis - in model organisms such as zebrafish and Drosophila melanogaster
3D cell culture - spheroids, tissue and organotypic culture
Imaging optically cleared samples - CLARITY, PARS, iDISCO etc.
Plants - Arabidopsis
Images top left to bottom right: Embryonic Kidney from rat, Ant head, Glomerulus from rat kidney following tissue expansion, Drangonfly head.