Cryo-Electron Microscopy

Cryo-electron microscopy (cryo-EM) is the EM methods of choice for visualization of hydrated proteins, viruses, cells and small organisms. The samples are plunge frozen in liquid ethane, preserved in amorphous ice and imaged under cryo-condition (liquid nitrogen conditions). Single particle 3D reconstruction methodology is used for structure biology studies. Cryo-electron tomography is a used to visualize the native structure of, macromolecular complexes, cellular organelles or compartments.

More information on the SciLifeLab Cryo-EM facility.