This technique combines the specificity and ability to image live processes in light microscopy with the higher resolution capability of the AFM. The combination creates a unique opportunity to study the interactions of biological molecules with cells at multiple dimensions by simultaneously monitoring the interactions and conformational changes taking place at the cell surface and within cellular milieu.
Mohan et al. Journal of Cell Science. 128, 979-91 (2015)
The platform provides technical basis for high resolution and real time imaging as well as the force measurements to probe specific interactions between the biological molecules in their natural environment. The AFM operating mechanism is based on sensing the specimen through the force between its surface and a sharp probe. This is why AFM produces high-resolution topographic and force measurements in aqueous and physiologically relevant environments without the need to stain or pre-treat the specimens.
The sequence of events in the amyloid assembly. Scale bars are 100 nm