CLEM combines the advantage of Light Microscopy and Electron Microscopy to study the same sample and gain new biological insights. While in Light Microscopy, specific proteins are labeled and identified to visualize their dynamics and interactions in fixed or living cells, Electron Microscopy serves to obtain the full context and high-resolution detail.
Francis et al. Journal of Cell Science. 15;128, 4183-95 (2015)
Transmission Electron Microscopy, TEM, is an imaging-technique to observe samples such as proteins, viruses, bacteria or ultrathin sections of cells and tissue (50-300nm). With TEM you are able to observe your samples at a higher resolution than light microscopy. The TEM uses electrons that are scattered differently depending on the electron density of the sample, these differences form your image.
Scanning electron microscopy, SEM, is an eminent microscopy technique for analyzing surface features of micro- and nanoscale, by creating high-resolution images from the interaction between electron beam and sample surface. The ease of sample preparation and microscope operation makes SEM a suitable tool for a wide range of applications in multidisciplinary sciences and industry. With the recent advancement in correlative light-electron and in-situ electron microscopy, SEM is a potential candidate for visualizing high-magnification fluorescent-labeled samples and samples’ response under stimulus in real time, respectively.