This course covers lectures and practical demonstrations in SEM and TEM techniques. The contents include principles of electron microscopy, specimen preparation, cryo-electron microscopy and correlative light-electron microscopy. The main focus will be on imaging biological samples, but the course may also be suitable for material scientists interested in high-resolution electron microscopy.
Lectures at the KBC Building and laboratory demonstrations at UCEM, Umeå University, Umeå
Short description: Cryo-electron tomography (cryo-ET) is a powerful structural technique. Its power comes from its versatility to study biological systems in vitro or in situ, i.e. within cells or tissue. It has the ability to reach uncharted regions in biological systems at high resolution. Advancements in microscopes, cameras, and computation make it now possible to determine sub-10 Å 3-dimensional structures of molecules directly within cells.
This four-and-a-half-day workshop will cover cryo-ET workflows from sample preparation on EM grids to data processing and analysis. The workshop will be a mixture of lectures and practical sessions. Participants will get hands-on experience in sample preparation on EM grids (micropatterning), sample freezing (plunge freezing and high-pressure freezing), cryo-correlative light and electron microscopy (cryo-CLEM), cryo-focused ion beam milling (cryo-FIB), cryo-TEM tiltseries data collection, and data processing and analysis (MotionCor2, IMOD, Dynamo).
It is compulsory that applicants have previous cryo-EM experience either with single-particle or already with cryo-tomography.
This workshop is supported by SciLifeLab, CryoNET and Umeå University and organized by the Umeå Centre for Electron Microscopy (UCEM).
The purpose of the course is to prepare and train cryoEM facility users in sample preparation methods, introduce users to the image data acquisition workflow, expand knowledge about cryoEM methods among researchers and show that everyone can learn how to use cryoEM for structural biology.
The course is open for facility users or potential facility users, such as PhD students, postdocs, and researchers within the life sciences who are curious and will profit from cryoEM skills. Swedish and international course participants are welcome. To attend, the course participants should be familiar with electron microscopy and structural biology.
The Advanced Light Microscopy (ALM) SciLifeLab node of NMI gives a two-week PhD-level course on four new imaging and fluorescence spectroscopy techniques: Depletion-based Super-Resolution Imaging, MINFLUX, Light-Sheet Imaging, and FCS-Methods. Do not miss this opportunity if you are interested in learning about bioimaging, from molecules up to live model organisms, using advanced light microscopy.
Dates: Monday October 14 – Friday October 25, 2024 Credits: 3 hp for PhD students Location: Gamma 3, SciLifeLab, Solna Registration deadline: September 30, 2024 Registration: stewen@kth.se
Welcome to this electron microscopy course at our NMI node UCEM in Umeå. The course covers lectures and practical demonstrations in SEM and TEM techniques. The contents include principles of electron microscopy, specimen preparation, cryo-electron microscopy and correlative light-electron microscopy. The main focus will be on imaging biological samples, but the course may also be suitable for material scientists interested in high-resolution electron microscopy.
The Advanced Light Microscopy (ALM) NMI node at SciLifeLab welcomes you to a KTH networking event where leading KTH scientists present novel 3D biological sample generation and visualisation applications.
Take the chance to join this Mass Spectrometry Imaging Zoom seminar on the 4th April, 15:00-16:00 (CET time). It will be given by Dr. Peter Weber from Lawrence Livermore National Laboratory, US. Dr. Weber is an expert in NanoSIMS imaging, as well as multiphoton microscopy and coherent Raman imaging. His research focus is microanalysis, microbial ecology, soil processes, elemental and isotopic tracers, and microbial and nuclear forensics. He has analyzed a broad range of materials, worked on methods to link microbial function and identity in complex microbial communities, and has studied microbial mineralization, symbiosis, predation, nitrogen fixation, carbon fixation, carbon cycling and phosphorus uptake. Dr. Weber is interested in correlated imaging and pushing the limits of compositional sensitivity, including developing methods to analyze viruses, cell membranes, and microarrays.
Title: “NanoSIMS studies from microbial ecology to nuclear forensics: Connecting microanalysis to the world”
The ZOOM seminar will be held 15:00-16:00 including discussion, then 16:00-16:30 will be a separate informal discussion time for those that want to stay.
Euro-BoImaging is pleased to announce that the second Open Call from the Horizon Europe-funded canSERV project is here! All user projects – ranging from basic discovery science to translational science and into personalised oncology on any type of cancer – are eligible. The total indicative funding volume of this call is 1 Million Euro across the entire canSERV consortium. Within this project, NMI and many other Euro-Bioimaging Nodes offer access to their expertise. Read more in the links below!
