SRM

 Super-Resolution fluorescence microscopy

(3 Hp, SK3514)

 

September 16th to October 4th 2019

 

The course aims to provide all participants with training in the fundamental theoretical and experimental aspects of super-resolution microscopy (Nobel Prize in 2014).

 

Scientific tutorials will introduce fundamental and advance aspects of super-resolution fluorescence microscopy. Experimental demo-session will further deepen the understanding and applicability of super-resolution microscopy.

 

As the name implies, super-resolution fluorescence microscopy is a field in which novel optical approaches and single molecule sensitive fluorescence methods are applied to answer question in cellular biology via nanoscale biological imaging.

 

The course will be carried out at the national Science for life laboratory in Solna

Syllabus

Lectures / seminars:

Introduction to available super-resolution fluorescence microscopy techniques

(e.g. STED/PALM/SIM/RESOLFT/DNA-PAINT etc.) and their applicability.

Demo-sessions on commercial super-resolution microscopy platforms.

 

Prerequisites

Registered as PhD-student and submission of access project proposal (link below).

Non KTH-students also need to fill in the KTH-admittance form (link below).

 

Requirements

Literature study (1 university credit)

Laboratory work (1 university credit)

Examination (1 university credit)

 

Required reading

Scientific articles (distributed)

 

Contact: Hans Blom, hblom@kth.se; Ilaria Testa, testa@kth.se

 

 

Last application date: 2019-08-30

Goals

After the course all participants should have acquired the following knowledge/skills:

 

  • Fundamentals of each super-resolution microscopy method
  • Practical implementation of each super-resolution technique
  • Necessary conditions regarding sample preparations
  • Initial acquaintance of using commercial super-resolution microscopes
  • Present and discuss own scientific work and approaches to apply super-resolution fluorescence microscopy to individual projects

Access project proposal form KTH-admittance form

 

Selection

In the case of an overbooked course (maximum 24 students) selection will be done on submitted project proposals (i.e. necessity of super-resolution imaging). The proposal form can be down loaded from below. When completed the form should be sent to hblom@kth.se no later than 2018-01-10

 

Course Time Table

 

Week 1: September 16 - September 20, 2019

Home studies on super-resolution microscopy (review articles)

Delivery of ‘want-to-know’ questions on each super-resolution technique

(RESOLFT, SIM, STED, STORM, PALM, DNA-PAINT)

 

Week 2: September 23 - September 27, 2019

Lecturing & discussion sessions, including demos with commercial microscopes. See course memo for details.

The lectures are part of the SciLifeLab-Symposium, see program below.

 

Week 3: September 30 - October 4, 2019

Examination – to pass the course each participant need to hand in a written project proposal where they motivate the use and suitability of super-resolution microscopy in their own research, with the know-how of pros and cons acquired during the course.

 

Course Memo can be down loaded here:

 

 

Exam (grades)

The super-resolution fluorescence microscopy course ends with a take-home exam (project proposal writing) where each PhD-student addresses how to apply or further develop one or several techniques in their own research. The grade of the course is either pass or fail.