SMLM

 

PALM - Photo-activated localization microscopy

Resolution

  • x, y ≈ 20 - 40 nm; z ≈ 90 - 100nm

 

Possible Fluorophores

  • One or two colors e.g. PA-GFP and PC-mEOS3.2 or similar photoactivated fluorescent proteins.

 

Possible samples

  • Fixed cells

 

PALM relies on fluorochomes that can be either photoactivated, photoconverted or reversibly photoswitched

 

STORM - Stochastic Optical Reconstruction Microscopy

Resolution

  • x, y ≈ 10 - 20 nm; z ≈ 70 - 90nm

 

Possible Fluorophores

  • One or two colors e.g. Alexa647 and Atto488, combined with special buffers for best photo-activation dynamics.

 

Possible samples

  • Fixed cells