Lightsheet

Light sheet microscopy

Light Sheet Fluorescence Microscopy permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.

The facility offers access to a commercially available Lightsheet Z.1 (Zeiss) microscope, allowing users to image live and optically cleared samples at unprecedented speeds. Collaborative project support is provided with sample mounting, image acquisitions, post-acquisition processing and analysis of the resulting data.

 

Services

Light Sheet microscopy - live, fixed and optically cleared samples

Sample preparation and mounting

Post-acquisition processing - Image stitching, multiview fusion and deconvolution

Data analysis - Arivis Vision 4D and Imaris 8.x

 

Equipment

Zeiss Light Sheet Z.1 including:

Dual side Illumination with pivot scan to reduce shadows and striping artifacts

CO2 and temperature control modules

Two sCMOS PCOedge cameras for simultanous dual colour acquisition

 

Applications

Morphogenesis, Embryogenesis and Organogenesis - in model organisms such as zebrafish and Drosophila melanogaster

3D cell culture - spheroids, tissue and organotypic culture

Imaging optically cleared samples - CLARITY, PARS, iDISCO etc.

Expansion microscopy

Plants - Arabidopsis

 

Images top left to bottom right: Embryonic Kidney from rat, Ant head, Glomerulus from rat kidney following tissue expansion, Drangonfly head.