Access to imaging technologies and expertise in the National Microscopy Infrastructure is provided by four NMI Nodes. The Nodes offer a powerful portfolio of different state-of-the-art technologies in the fields of biological imaging. Each Node brings in a different combination of technologies as well as their expertise with distinct applications and methods.
Multimodal assimilation of technologies provides complementary and often unique information, spatial, structural, biochemical and biophysical details from the same target sample.
Singel molecule localization microsopy
Within the NMI infrastructure we have the equipment and experience to performe single molecule localization microscopy with both PALM and STORM.
CLM-AFM - Correlative Light and Atomic Force Microscopy
Within the NMI infrastructure we have the equipment and experience to performe CLM-AFM
High throughput confocal microscopy
The NMI infrastructure enables large number of fixed samples to be imaged in 96 or 384 plate format using laser scanning confocal microscopy.
CLEM - Correlative light electron microscopy
Within NMI we have the equipment and experience to performe CLEM using transmission electron microscopy (TEM) and scanning electon microscopy (SEM)
IVM - Intravital microscopy
NMI offer a broad range of intravital microscopy applications with multi- and/or single-photon microscopy and FLIM.
FCS/FCCS - Fluorescence correlation spectroscopy
Measures diffusion coefficients and concentrations of fluorescent molecules in solution and in living cells by monitoring fluorescence intensity fluctuations in a single spot.
RESOLFT - Reversible Saturable Optical Fluorescence Transitionsmicroscopy
The RESOLFT technique is currently under development at the KTH node.
More information to come.
Light sheet microscopy
Light Sheet Fluorescence Microscopy permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.