The NMI facility for intravital microscopy (IVM) is currently under construction at Stockholm University. This open access facility called IVMSU will be incorporated in a new research laboratory for studies of living rodents (mice and rats). It will be equipped for a broad range of IVM-applications that require both multi- and single-photon microscopy for analysis of processes at cellular and subcellular levels at high resolution in the context of whole animals, exteriorized organs, skin flaps, fruit fly embryos and even single cells.
Nearby to IVMSU, the existing Imaging Facility at Stockholm University (IFSU) runs a well-organized microscopy unit for different common imaging techniques used by researchers in the area of molecular biosciences. Together with IFSU and the new animal laboratory, IVMSU will form an advanced open access research facility with unique potential for scientists to perform future studies of cellular processes in cells and tissues of living rodents.
IVMSU will house both an inverted and an up-right microscope, connected to a state-of-the art multi-photon laser with fixed and tunable laser lines (680-1300 nm). Hence also label free modalities such as SHG and THG will be possible. In addition, a range of single-photon laser lines will be available, and detectors and scanners will be of the best currently present on the market. Both stages will be built for observation of anesthetized animals as well as insects and cells under temperature controlled conditions. For the latter, IVMSU is expected to function as a “top-end” complement to the single photon laser scanning imaging available at IFSU.
A specially designed and equipped surgery and an animal housing room ensure that users will be able carry out their studies under ethically approved conditions and perform experiments involving repeated microscopy sessions over extended periods of time. As an open-access facility this makes IVMSU unique also in a European perspective.
The construction works are currently ahead of schedule, and instrument installations and testing are expected to be performed during Oct-Dec this year with the possibility to allow the first users to enter in January 2018.
IVMSU are accepting projects to start during spring 2018