GU

Project Coordinator

Julia Fernandez-Rodriguez - juliafer@cci.sahlgrenska.gu.se

University of Gothenburg - Centre for Cellular Imaging - Multimodal Advanced Microscopy

 

The Centre for Cellular Imaging (CCI) is a core facility that integrate both Light and Electron microscopy. Our vision is that the CCI will allow users to think broadly and select the best possible instrument(s), light and/or electron microscope(s), to provide detailed answers to their scientific questions at the highest resolution. This multi-modal assimilation of technologies provides complementary and often unique information, spatial, structural, biochemical and biophysical details from the same target sample. We are committed to developing a functional systems microscopy approach, which can address new scientific questions with a multi-modal imaging strategy using only a few or many samples. Using modern cell biological tools, expertise in live cell imaging and state-of-the-art equipment and image analysis we will continue to help researchers relate structure to function and morphology to mechanism.

The Centre for Cellular Imaging (CCI) provides many technologies and methods for life science research. The majority of imaging systems can be booked online with hourly billing after on-site training by facility staffs

 

Two categories of technologies are available within the CCI:

 

  • Advanced Microscopy Imaging Systems (including regular and automated wide field microscopy, Laser Scanning confocal microscopes, Multiphoton, TEM and SEM etc…), that you can consult by the website of the CCI
  • Sophisticated and Innovative Imaging Systems, technologies and methods are also accessible. They are categorized underneath.

 

Innovative Imaging Systems

 

High-Resolution and single particle tracking:

 

  • 3D Super-resolution Structured illumination microscopy (SIM)

 

  • 3D Single Molecule Localization Microscopy (PALM, STORM, TIRF)

 

  • Airyscan

 

Fast Laser Scanning Microscopy: LSM 880 Airyscan with the Fast Mode

 

Non-linear microscopy: Multiphoton, Second and Third Harmoic Generation

 

Confocal with innovative modalities:

 

  • Fluorescence Resonance Energy Transfer (FRET)

 

  • Fluorescence Recovery After Photobleaching (FRAP)

 

  • Fluorescence Correlation Spectroscopy (FCS)

 

  • Fluorescen Cross Correlation Spectroscopy (FCCS)

 

Correlative Light Electron Microscopy (CLEM)

 

Laser Microdissection and Pressure Catapulting (LMPC)